en بررسی گیرنده ‌های شبه Toll 2، 4 و 9 محلول و مولکول‌ها DAMPS سیگنال‌دهنده آن‌ها(HMGB1, HSP70) در بیماران مبتلا به سرطان پستان استان بصره پژوهشي Research <div style="text-align: justify;"><span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">مقدمه:</span></span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> گیرنده&rlm; های</span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> شبه </span></span><span dir="LTR" style="font-size:11.0pt">Toll</span> <span dir="LTR" style="font-size:11.0pt">(TLR)</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">، </span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">اعضای گیرنده‌های تشخیص الگو هستند که انواع مولکول‌ها شامل الگوهای مولکولی مرتبط با بیماری‌زا (</span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">PAMPs</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">)</span></span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">و </span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">الگوهای مولکولی مرتبط با آسیب (</span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">DAMPs</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">) </span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">را شناسایی می‌کنند.</span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> این گیرنده &rlm;ها توسط سلول&rlm; های ایمنی، غیرایمنی و توموری نیز بیان می&rlm;شوند. برخی از&rlm;</span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">TLR </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">ها در پیشرفت تومور و برخی دیگر در سرکوب تومور نقش دارند. هدف مطالعه ما ارزیابی</span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">TLR۲</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">، </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">TLR۴</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">، </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">TLR۹</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">، </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">HMGB۱</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> و</span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black"> HSP۷۰ </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">محلول در سرم بیماران مبتلا به سرطان پستان برای درک </span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">بهتر </span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نقش آن&rlm;ها در توسعه سرطان بود.</span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black"></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">مواد و روش‌ها:</span></span></span></b><i> </i><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">جامعه مورد مطالعه شامل ۵۰ بیمار مبتلا به سرطان پستان و ۲۵ فرد سالم بود. پس از کسب رضایت آگاهانه از شرکت&rlm; کنندگان واجد شرایط ورود به مطالعه، از هر فرد </span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">۳ میلی&rlm;لیتر خون وریدی <span style="color:black">گرفته و پس از سانتریفیوز،</span></span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نمونه سرم‌های جمع &rlm;آوری شده با استفاده از روش ایمونوسوربنت متصل به آنزیم (</span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">ELISA</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">)</span></span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">مورد آزمایش قرار گرفت و میزان سرمی گیرنده&rlm; های فوق تعیین شد و مورد مقایسه قرار گرفت.</span></span></span><span lang="AR-SA" style="font-size:11.0pt"></span></span></span></span></span><br> <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">یافته&nbsp;</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">&rlm;ها</span></span></b><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">: نتایج به&rlm;دست آمده از بررسی سرم افراد نشاد داد که سطح سرمی </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">TLR۲</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">، </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">TLR۹</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">،</span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">HMGB۱ </span></span><span style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">&nbsp;و</span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black"> HSP۷۰ </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">محلول، در سرم بیماران به طور قابل توجهی بالاتر از افراد سالم بود. همچنین مشخص شد که سطح سرمی</span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black"> TLR۹ </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">و</span></span></span> <span dir="LTR" style="font-size:11.0pt"><span style="color:black">HMGB۱</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">در مرحله </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">II</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">&nbsp;IV</span></span><span style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> بیماری </span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">بالاتر است.</span></span></span><b><span lang="AR-SA" style="font-size:11.0pt"></span></b></span></span></span></span><br> <b><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نتیجه</span></span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">&rlm;گیری: </span></span></b><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">نتایج این مطالعه نشان داد که</span></span> <span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">سطح سرمی </span></span><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">TLR</span></span></span><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">۹</span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> محلول و&nbsp;</span></span><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">HMGB۱ </span></span><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">&nbsp;ممکن است یک عامل پیش‌آگهی جدید در سرطان پستان باشند و می‌توانند به عنوان اهداف احتمالی جهت ایمونوتراپی در آینده در نظر گرفته شوند.</span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">اگرچه جهت معرفی این موضوع به حیطه بالینی، به شواهد بیشتری نیاز است.</span></span></div> <div style="text-align: justify;"><span style="font-size:9pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span style="line-height:115%">Introduction:</span></span></b> <span style="font-size:11.0pt"><span style="line-height:115%">Toll-Like Receptors (TLRs)<b> </b>are members of pattern recognition receptors that recognize various molecules, including pathogen-associated molecular patterns and dead-associated molecular patterns. These receptors are expressed by immune, non-immune, and tumor cells. Some TLRs are implicated in tumor progression, while others are involved in tumor suppression. Our study aimed to evaluate soluble TLR2, TLR4, TLR9, HMGB1,<i> </i>and HSP70<i> </i>in breast cancer patients&#39; sera to further understand their roles in breast cancer development.</span></span><span style="font-size:11.0pt"><span style="line-height:115%"></span></span></span></span></span><br> <span style="font-size:9pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span style="line-height:115%">Methods:</span></span></b><span style="font-size:11.0pt"><span style="line-height:115%"> The study population includes 50 breast cancer patients and 25 healthy individuals</span></span><i><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:">. </span></span></span></i><span style="font-size:11.0pt"><span style="line-height:115%">Patients provided written informed consent before</span></span><i> </i><span style="font-size:11.0pt"><span style="line-height:115%">participating in the study</span></span><i><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:">. </span></span></span></i><span style="font-size:11.0pt"><span style="line-height:115%">Three milliliters of venous blood were drawn from each person, and serum samples were evaluated for Toll-Like Receptors (TLRs) titer by ELISA assay.</span></span></span></span></span><br> <span style="font-size:9pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span style="line-height:115%">Results:</span></span></b><span style="font-size:11.0pt"><span style="line-height:115%"> Soluble TLR2, TLR9, HMGB1, and HSP70 were significantly higher in patients&#39; serum than in healthy controls. It was shown that the serum levels of TLR9 and HMGB1 were more elevated in stages II and IV of the disease.</span></span><b><span style="font-size:11.0pt"><span style="line-height:115%"></span></span></b></span></span></span><br> <span style="font-size:9pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span style="line-height:115%">Conclusion:</span></span></b><span style="font-size:11.0pt"><span style="line-height:115%"> Our results suggest that soluble TLR2, 9, and HMGB1 may be a novel prognostic factor in breast cancer, and they could be a target for immunotherapy in the future. However, more evidence is needed before introducing this to the bedside.</span></span></span></span></span></div> سرطان پستان, گیرنده, Toll-like, HMGB1, HSP70 reast Cancer, Toll-Like Receptors, HSP70, HMGB1 50 62 http://ijbd.ir/browse.php?a_code=A-10-1049-1&slc_lang=en&sid=1 Noor Fadhil Abdulabbas نور فدهیل عبدالعباس noorfadhil_89@yahoo.com 00031947532846008881 00031947532846008881 Yes Department of Biology, Colleg of Science, University of Basrah, Basrah, Iraq گروه بیولوژی، دانشکده علوم، دانشگاه بصره، بصره، عراق Wafaa Sadoon Shani وفا سادون شانی Wafaa_Sadoon@yahoo.com 00031947532846008882 00031947532846008882 No Department of Biology, Colleg of Science, University of Basrah, Basrah, Iraq گروه بیولوژی، دانشکده علوم، دانشگاه بصره، بصره، عراق